Hotspot

rpl32-trnL-UAG

SSC · 117884-119319 · 1436 bp

Back to Hedlundia

Pi 0.0065
MarkerSeek score 42.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1436
variable_sites
25
pi
0.0065
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1548-1634 bp 1.0 82.7
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1549-1635 bp 1.0 82.7
rpl32-trnL-UAG_p3 TAGGGCAGCATTGAAAGCTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1550-1636 bp 1.0 82.3
rpl32-trnL-UAG_p4 GGGCAGCATTGAAAGCTTTT ATCCACACAAAAGAGCTGCA 45.0 45.0 57.8 58.0 2159-2239 bp 1.0 81.8
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT TCCACACAAAAGAGCTGCAT 45.0 45.0 57.8 58.0 2158-2238 bp 1.0 81.8