Hotspot

trnR-UCU-atpA

LSC · 10163-10841 · 679 bp

Back to Hedlundia

Pi 0.017

MarkerSeek score 51.10

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 679
variable_sites: 28
pi: 0.017
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	ACAATCTCAATCATCCCCGC	50.0	50.0	58.0	58.0	963-1337 bp	1.0	85.5
trnR-UCU-atpA_p2	CCTAGCCTTCCAAGCTAACG	ACAATCTCAATCATCCCCGC	55.0	50.0	58.1	58.0	966-1340 bp	1.0	85.3
trnR-UCU-atpA_p3	AGCCTTCCAAGCTAACGATG	TTCGGTTTCCAGGGTAGGAT	50.0	50.0	58.0	58.0	1143-1517 bp	1.0	85.2
trnR-UCU-atpA_p4	AGCCTTCCAAGCTAACGATG	TCAGACCGGCCATTAATGTG	50.0	50.0	58.0	58.0	1168-1542 bp	1.0	85.1
trnR-UCU-atpA_p5	CCTAGCCTTCCAAGCTAACG	TCAGACCGGCCATTAATGTG	55.0	50.0	58.1	58.0	1171-1545 bp	1.0	84.9