Hotspot

psbA-trnK-UUU

LSC · 1515-1790 · 276 bp

Back to Hedlundia

Pi 0.00797

MarkerSeek score 55.20

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 276
variable_sites: 6
pi: 0.00797
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TCCAGTTACAAAAGCGACCC	CGGTTTGGGGAGGGATTTTT	50.0	50.0	58.1	58.4	422-436 bp	1.0	79.9
psbA-trnK-UUU_p2	TCCAGTTACAAAAGCGACCC	AAAATGCAAGCACGGTTTGG	50.0	45.0	58.1	58.4	434-448 bp	1.0	79.3
psbA-trnK-UUU_p3	TCCAGTTACAAAAGCGACCC	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.1	58.6	365-379 bp	1.0	78.5
psbA-trnK-UUU_p4	ACGGTTTTCAGTGCTGGTTA	CGGTTTGGGGAGGGATTTTT	45.0	50.0	57.7	58.4	442-456 bp	1.0	78.0
psbA-trnK-UUU_p5	TCCAGTTACAAAAGCGACCC	TGAAAAATGCAAGCACGGTT	50.0	40.0	58.1	57.4	437-451 bp	1.0	77.9