Hotspot

ycf1

SSC · 126510-132140 · 5631 bp

Back to Hedera

Pi 0.000533
MarkerSeek score 52.90
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5631
variable_sites
6
pi
0.000533
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TGTTGATTCAAGAACAAGTTGTGT AATGTAGAATCGCCAAAAGA 33.3 35.0 57.7 52.9 177 bp 1.0 40.9
ycf1_p2 TGTTGATTCAAGAACAAGTTGTGT ACAAGCACAATCAGAAAGAA 33.3 35.0 57.7 53.3 270 bp 1.0 40.6
ycf1_p3 TGTTGATTCAAGAACAAGTTGTGT ACAAGCACAATCAGAAAGAAA 33.3 33.3 57.7 54.2 270 bp 1.0 40.6
ycf1_p4 TGTTGATTCAAGAACAAGTTGTGT ACAAGCACAATCAGAAAGAAAA 33.3 31.8 57.7 55.0 270 bp 1.0 40.6
ycf1_p5 TGTTGATTCAAGAACAAGTTGTG ACAAGCACAATCAGAAAGAA 34.8 35.0 56.2 53.3 270 bp 1.0 40.6