Hotspot

rps16

LSC · 5230-6356 · 1127 bp

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Pi 0.000592
MarkerSeek score 27.00
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1127
variable_sites
1
pi
0.000592
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
27.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 TTCGTTTGATTAGGGCAAAG TGTACCGACTGAACCAATGAC 40.0 47.6 54.2 58.0 1231-1232 bp 1.0 41.4
rps16_p2 TTTCGTTTGATTAGGGCAAAG TGTACCGACTGAACCAATGAC 38.1 47.6 55.0 58.0 1232-1233 bp 1.0 40.1
rps16_p3 TTCGTTTGATTAGGGCAAAG GTACCGACTGAACCAATGACT 40.0 47.6 54.2 57.7 1230-1231 bp 1.0 40.1
rps16_p4 TTTCGTTTGATTAGGGCAAAG GTACCGACTGAACCAATGACT 38.1 47.6 55.0 57.7 1231-1232 bp 1.0 40.1
rps16_p5 TTCGTTTGATTAGGGCAAAG TGTACCGACTGAACCAATGA 40.0 45.0 54.2 56.5 1231-1232 bp 1.0 40.1