Hotspot

rpoC1

LSC · 22193-25022 · 2830 bp

Back to Hedera

Pi 0.000648
MarkerSeek score 44.40
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
LSC
length_bp
2830
variable_sites
3
pi
0.000648
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 ACTTAGCTTAGGTACCATCTGA TTGCTCGTTCGAGAACTACG 40.9 50.0 55.7 58.0 2948-2954 bp 1.0 45.7
rpoC1_p2 ACTTAGCTTAGGTACCATCTGA ACGATCTTTGGCTCTGGAAC 40.9 50.0 55.7 57.9 2931-2937 bp 1.0 45.2
rpoC1_p3 ACTTAGCTTAGGTACCATCTGA TTCGATTGCTCGTTCGAGAA 40.9 45.0 55.7 57.7 2953-2959 bp 1.0 43.3
rpoC1_p4 ACTTAGCTTAGGTACCATCTGA TTTCGATTGCTCGTTCGAGA 40.9 45.0 55.7 57.7 2954-2960 bp 1.0 43.3
rpoC1_p5 ACTTAGCTTAGGTACCATCTGA CGATTGCTCGTTCGAGAACT 40.9 50.0 55.7 58.4 2951-2957 bp 1.0 43.0