Hotspot

ndhF-rpl32

SSC · 114744-115922 · 1179 bp

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Pi 0.00127
MarkerSeek score 50.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1179
variable_sites
3
pi
0.00127
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG TAAGGCTGCCCAATATCCCT 50.0 50.0 58.0 58.2 1364 bp 1.0 78.6
ndhF-rpl32_p2 TGCCGTCGGAAAAAGAAGAA TAAGGCTGCCCAATATCCCT 45.0 50.0 58.0 58.2 1362 bp 1.0 78.5
ndhF-rpl32_p3 TTGCCGTCGGAAAAAGAAGA TAAGGCTGCCCAATATCCCT 45.0 50.0 58.0 58.2 1363 bp 1.0 78.5
ndhF-rpl32_p4 GTTGCCGTCGGAAAAAGAAG GACAACGCTTTTAAGGCTGC 50.0 50.0 58.0 58.3 1375 bp 1.0 77.4
ndhF-rpl32_p5 TGCCGTCGGAAAAAGAAGAA GACAACGCTTTTAAGGCTGC 45.0 50.0 58.0 58.3 1373 bp 1.0 77.3