Hotspot

trnP-UGG-psaJ

LSC · 68997-69382 · 386 bp

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Pi 0.00302
MarkerSeek score 38.40
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
386
variable_sites
2
pi
0.00302
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC TGTCAACGCATCTGGGAAAA 50.0 45.0 58.1 58.0 555 bp 1.0 79.3
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA TGTCAACGCATCTGGGAAAA 50.0 45.0 58.1 58.0 554 bp 1.0 79.3
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC TGTCAACGCATCTGGGAAAA 50.0 45.0 58.1 58.0 566 bp 1.0 79.2
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA TGTCAACGCATCTGGGAAAA 50.0 45.0 58.1 58.0 565 bp 1.0 79.2
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG TGTCAACGCATCTGGGAAAA 50.0 45.0 58.1 58.0 568 bp 1.0 79.2