Hotspot

accD-psaI

LSC · 61279-61977 · 699 bp

Back to Hedera

Pi 0.00215
MarkerSeek score 50.90
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
699
variable_sites
3
pi
0.00215
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GCTCCACGCTTTCTTTCCTT AAAGAAGCCATTGCCATTGC 50.0 45.0 58.8 57.9 804-806 bp 1.0 73.4
accD-psaI_p2 GCTCCACGCTTTCTTTCCTT TTGCCATTGCCGGAAATACT 50.0 45.0 58.8 58.2 794-796 bp 1.0 73.0
accD-psaI_p3 GCTCCACGCTTTCTTTCCTT TAAAGGCACCAACACAGAGG 50.0 50.0 58.8 57.7 766-768 bp 1.0 72.1
accD-psaI_p4 GCTCCACGCTTTCTTTCCTT CACCAACACAGAGGGAAAGT 50.0 50.0 58.8 57.7 760-762 bp 1.0 71.4
accD-psaI_p5 GCTCCACGCTTTCTTTCCT AAAGAAGCCATTGCCATTGC 52.6 45.0 58.1 57.9 804-806 bp 1.0 70.9