Hotspot

ycf3-trnS-GGA

LSC · 46814-47676 · 863 bp

Back to Hedera

Pi 0.00174
MarkerSeek score 45.90
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
863
variable_sites
3
pi
0.00174
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTGGGAA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.3 58.1 1026-1048 bp 1.0 65.8
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTGGGA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.3 58.1 1027-1049 bp 1.0 65.8
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTGGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.3 58.2 1047-1069 bp 1.0 65.3
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTGGGA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.3 58.2 1048-1070 bp 1.0 65.3
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTGGGAA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.3 57.8 997-1019 bp 1.0 65.0