Hotspot

petN-psbM

LSC · 30385-31264 · 880 bp

Back to Hedera

Pi 0.00133
MarkerSeek score 51.90
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
880
variable_sites
2
pi
0.00133
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA AGTTCCTACTGCCTTTCTGC 45.0 50.0 58.0 57.5 1002-1017 bp 1.0 77.4
petN-psbM_p2 TCTTGCTTGGGCTGCTTTAA GTTCCTACTGCCTTTCTGCT 45.0 50.0 58.0 57.5 1001-1016 bp 1.0 77.4
petN-psbM_p3 AGTAAGTCTTGCTTGGGCTG AGTTCCTACTGCCTTTCTGC 50.0 50.0 57.8 57.5 1008-1023 bp 1.0 76.0
petN-psbM_p4 AGTAAGTCTTGCTTGGGCTG GTTCCTACTGCCTTTCTGCT 50.0 50.0 57.8 57.5 1007-1022 bp 1.0 76.0
petN-psbM_p5 ATGGGGAAGAAGTGGACTCT GTTCCTACTGCCTTTCTGCT 50.0 50.0 57.6 57.5 947-962 bp 1.0 74.8