Hotspot

trnS-CGA-trnR-UCU

LSC · 10964-11188 · 225 bp

Back to Hedera

Pi 0.00246
MarkerSeek score 47.50
Primer available yes
Label trnS-CGA-trnR-UCU

Feature Metadata

label_name
trnS-CGA-trnR-UCU
feature_type
igs
region
LSC
length_bp
225
variable_sites
1
pi
0.00246
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.90
markerseek_score
47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-CGA-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 284-306 bp 1.0 67.6
trnS-CGA-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 287-309 bp 1.0 67.4
trnS-CGA-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 290-312 bp 1.0 67.0
trnS-CGA-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.1 57.9 293-315 bp 1.0 66.7
trnS-CGA-trnR-UCU_p5 CCCTAGCCTTCCAAGCTAAC AGAAGACCTCTGTCCTATCCA 55.0 47.6 57.7 56.9 288-310 bp 1.0 65.2