Hotspot

rpl22

LSC · 129125-129511 · 387 bp

Back to Hechtia

Pi 0.00647

MarkerSeek score 37.00

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 387
variable_sites: 9
pi: 0.00647
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CGTGCAATTCCTTCGAAACC	TCTGACTTTCGCGAGACATG	50.0	50.0	58.0	58.0	756-768 bp	1.0	84.1
rpl22_p2	CGTGCAATTCCTTCGAAACC	TAGTAACTTGGTCTCGGGCA	50.0	50.0	58.0	58.1	895-907 bp	1.0	83.0
rpl22_p3	CGTGCAATTCCTTCGAAACC	GTTATCCTGCGCTTGGAAGA	50.0	50.0	58.0	58.0	1320-1331 bp	1.0	82.4
rpl22_p4	TCGATACGGCCTGCTATTTG	TCTGACTTTCGCGAGACATG	50.0	50.0	57.9	58.0	1077-1089 bp	1.0	82.2
rpl22_p5	CGTGCAATTCCTTCGAAACC	GATCCACTTGGCTACATCCG	50.0	55.0	58.0	58.1	1147-1158 bp	1.0	81.9