Hotspot

ndhD

SSC · 4064-5586 · 1523 bp

Back to Hechtia

Pi 0.00562

MarkerSeek score 65.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1523
variable_sites: 42
pi: 0.00562
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAGACTTTCCTTCCGTCTCA	TTTTGAGGACGGGCTTTTCT	45.0	45.0	56.1	57.6	1437-1586 bp	1.0	68.2
ndhD_p2	AAGACTTTCCTTCCGTCTCA	TTTGAGGACGGGCTTTTCTG	45.0	50.0	56.1	58.4	1436-1585 bp	1.0	67.9
ndhD_p3	AAGACTTTCCTTCCGTCTCA	TCTTTACCGACAAAAGCCCG	45.0	50.0	56.1	58.5	1476-1625 bp	1.0	67.1
ndhD_p4	AGACTTTCCTTCCGTCTCAT	TTTTGAGGACGGGCTTTTCT	45.0	45.0	55.9	57.6	1436-1585 bp	1.0	66.3
ndhD_p5	AGACTTTCCTTCCGTCTCAT	TTTGAGGACGGGCTTTTCTG	45.0	50.0	55.9	58.4	1435-1584 bp	1.0	66.0