Hotspot

rpl22-rps19

IRa · 129512-129613 · 102 bp

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Pi 0.0294

MarkerSeek score 65.50

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRa
length_bp: 102
variable_sites: 10
pi: 0.0294
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GCCCGCGAATCTGATCAATT	TCTGACTTTCGCGAGACATG	50.0	50.0	59.1	58.0	253-260 bp	1.0	76.5
rpl22-rps19_p2	GCCCGCGAATCTGATCAATT	CCTACTCTGACTTTCGCGAG	50.0	55.0	59.1	57.8	258-265 bp	1.0	75.1
rpl22-rps19_p3	GCCCGCGAATCTGATCAATT	CTCTGACTTTCGCGAGACAT	50.0	50.0	59.1	57.7	254-261 bp	1.0	74.4
rpl22-rps19_p4	GCCCGCGAATCTGATCAAT	TCTGACTTTCGCGAGACATG	52.6	50.0	58.4	58.0	253-260 bp	1.0	73.9
rpl22-rps19_p5	GCCCGCGAATCTGATCAATT	CAAATTGGGAGAATTCGCGC	50.0	50.0	59.1	58.5	277-284 bp	0.928571	69.7