Hotspot

rps3-rpl22

LSC · 129085-129124 · 40 bp

Back to Hechtia

Pi 0.0326

MarkerSeek score 67.90

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 40
variable_sites: 7
pi: 0.0326
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CGTGCAATTCCTTCGAAACC	AAAGCCGAAGCCAATAGGAG	50.0	50.0	58.0	58.0	366-375 bp	1.0	85.0
rps3-rpl22_p2	CGTGCAATTCCTTCGAAACC	TCTGACTTTCGCGAGACATG	50.0	50.0	58.0	58.0	756-768 bp	1.0	84.1
rps3-rpl22_p3	CGTGCAATTCCTTCGAAACC	TAGTAACTTGGTCTCGGGCA	50.0	50.0	58.0	58.1	895-907 bp	1.0	83.0
rps3-rpl22_p4	TCGATACGGCCTGCTATTTG	TCTGACTTTCGCGAGACATG	50.0	50.0	57.9	58.0	1077-1089 bp	1.0	82.2
rps3-rpl22_p5	GTCGATACGGCCTGCTATTT	TCTGACTTTCGCGAGACATG	50.0	50.0	57.8	58.0	1078-1090 bp	1.0	82.2