Hotspot

ndhC-trnV-UAC

LSC · 94807-95858 · 1052 bp

Back to Hechtia

Pi 0.00835

MarkerSeek score 72.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1052
variable_sites: 40
pi: 0.00835
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAACTCC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	1148-1211 bp	1.0	85.0
ndhC-trnV-UAC_p2	ACACTAACCGGGGCTAAAAC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1151-1214 bp	1.0	83.7
ndhC-trnV-UAC_p3	CGGGGCTAAAACTCCTGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1143-1206 bp	1.0	83.7
ndhC-trnV-UAC_p4	TAACCGGGGCTAAAACTCCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	1147-1210 bp	1.0	82.5
ndhC-trnV-UAC_p5	ACTAACACTAACCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1155-1218 bp	0.785714	73.4