Hotspot

petN-psbM

LSC · 74335-75543 · 1209 bp

Back to Hechtia

Pi 0.00557
MarkerSeek score 65.10
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1209
variable_sites
34
pi
0.00557
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GTATGGGGAAGGAGTGGACT TGCTACTGCACTGTTCATTCT 55.0 42.9 58.1 57.6 1292-1374 bp 1.0 71.3
petN-psbM_p2 AGTATGGGGAAGGAGTGGAC TGCTACTGCACTGTTCATTCT 55.0 42.9 58.1 57.6 1293-1375 bp 1.0 71.2
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1351-1433 bp 1.0 70.9
petN-psbM_p4 TATGGGGAAGGAGTGGACTC TGCTACTGCACTGTTCATTCT 55.0 42.9 57.8 57.6 1291-1373 bp 1.0 70.8
petN-psbM_p5 GGGGAAGGAGTGGACTCTAG TGCTACTGCACTGTTCATTCT 60.0 42.9 58.2 57.6 1288-1370 bp 1.0 70.5