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trnC-GCA-petN

LSC · 73210-74244 · 1035 bp

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Pi 0.00726

MarkerSeek score 66.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1035
variable_sites: 42
pi: 0.00726
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	AGTTCAAATCTGGGTGTCGC	AGTCCACTCCTTCCCCATAC	50.0	55.0	58.5	58.1	1140-1166 bp	1.0	79.6
trnC-GCA-petN_p2	AGTTCAAATCTGGGTGTCGC	GTCCACTCCTTCCCCATACT	50.0	55.0	58.5	58.1	1139-1165 bp	1.0	79.6
trnC-GCA-petN_p3	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	1081-1107 bp	1.0	79.5
trnC-GCA-petN_p4	AGTTCAAATCTGGGTGTCGC	GAGTCCACTCCTTCCCCATA	50.0	55.0	58.5	57.8	1141-1167 bp	1.0	79.1
trnC-GCA-petN_p5	AGTTCAAATCTGGGTGTCGC	CTAGAGTCCACTCCTTCCCC	50.0	60.0	58.5	58.2	1144-1170 bp	1.0	78.8