Hotspot

rpoB-trnC-GCA

LSC · 71767-73138 · 1372 bp

Back to Hechtia

Pi 0.00644

MarkerSeek score 71.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1372
variable_sites: 47
pi: 0.00644
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATTCCGGA	AAAGGATTTGCAGTCCCCTG	50.0	50.0	58.4	58.1	1172-1456 bp	1.0	82.8
rpoB-trnC-GCA_p2	TTCCCTCATTTCCATTCCGG	AAAGGATTTGCAGTCCCCTG	50.0	50.0	57.6	58.1	1173-1457 bp	1.0	82.6
rpoB-trnC-GCA_p3	TCCCTCATTTCCATTCCGGA	GCGACACCCAGATTTGAACT	50.0	50.0	58.4	58.5	1198-1482 bp	1.0	79.5
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATTCCGG	GCGACACCCAGATTTGAACT	50.0	50.0	57.6	58.5	1199-1483 bp	1.0	79.3
rpoB-trnC-GCA_p5	TCTTCCGTCAAGCCTTGATT	AAAGGATTTGCAGTCCCCTG	45.0	50.0	57.1	58.1	1249-1533 bp	1.0	79.1