Hotspot

rps19-psbA

LSC · 43650-43786 · 137 bp

Back to Hechtia

Pi 0.0342

MarkerSeek score 70.10

Primer available yes

Label rps19-psbA

Feature Metadata

label_name: rps19-psbA
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 14
pi: 0.0342
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-psbA_p1	TCTGACTTTCGCGAGACATG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.0	57.9	230-237 bp	1.0	84.9
rps19-psbA_p2	TCTGACTTTCGCGAGACATG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.0	57.9	233-240 bp	1.0	84.7
rps19-psbA_p3	CCTACTCTGACTTTCGCGAG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	57.8	57.9	235-242 bp	1.0	83.4
rps19-psbA_p4	CCTACTCTGACTTTCGCGAG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	57.8	57.9	238-245 bp	1.0	83.3
rps19-psbA_p5	CTCTGACTTTCGCGAGACAT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.7	57.9	231-238 bp	1.0	82.8