Hotspot

atpD

LSC · 1463-2026 · 564 bp

Pi 0.0496

MarkerSeek score 84.90

Primer available yes

Label atpD

Feature Metadata

label_name: atpD
feature_type: gene
region: LSC
length_bp: 564
variable_sites: 42
pi: 0.0496
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpD_p1	TTTCTCAATTTGTTCACGGA	GGACCAAAAGAACGTGCAAC	35.0	50.0	53.4	58.2	731 bp	1.0	46.1
atpD_p2	TTTCTCAATTTGTTCACGGA	ACAGTTGTTCGTGCACAAGA	35.0	45.0	53.4	58.3	758 bp	1.0	45.1
atpD_p3	TTCTCAATTTGTTCACGGAT	GGACCAAAAGAACGTGCAAC	35.0	50.0	53.1	58.2	730 bp	1.0	44.6
atpD_p4	ATTTCTCAATTTGTTCACGGA	GGACCAAAAGAACGTGCAAC	33.3	50.0	54.0	58.2	732 bp	1.0	44.6
atpD_p5	TTTCTCAATTTGTTCACGGAT	GGACCAAAAGAACGTGCAAC	33.3	50.0	54.0	58.2	731 bp	1.0	44.6