Hotspot

OOD24_pgt026-clpC

SSC · 101179-101314 · 136 bp

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Pi 0.13
MarkerSeek score 81.80
Primer available yes
Label OOD24_pgt026-clpC

Feature Metadata

label_name
OOD24_pgt026-clpC
feature_type
igs
region
SSC
length_bp
136
variable_sites
24
pi
0.13
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
OOD24_pgt026-clpC_p1 CCTTACCACTTGGCCATACC CCCATTCTACGGGCTTCTTC 55.0 55.0 57.9 58.0 200-230 bp 1.0 91.2
OOD24_pgt026-clpC_p2 CTTACCACTTGGCCATACCG CCCATTCTACGGGCTTCTTC 55.0 55.0 58.3 58.0 199-229 bp 1.0 88.9
OOD24_pgt026-clpC_p3 TTGCAATCCACTGCCTTACC CCCATTCTACGGGCTTCTTC 50.0 55.0 58.5 58.0 213-243 bp 1.0 88.0
OOD24_pgt026-clpC_p4 ATCCACTGCCTTACCACTTG CCCATTCTACGGGCTTCTTC 50.0 55.0 57.5 58.0 208-238 bp 1.0 87.7
OOD24_pgt026-clpC_p5 CAATCCACTGCCTTACCACT CCCATTCTACGGGCTTCTTC 50.0 55.0 57.5 58.0 210-240 bp 1.0 87.7