Hotspot

dnaK-rpl3

SSC · 84618-84849 · 232 bp

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Pi 0.114
MarkerSeek score 80.80
Primer available yes
Label dnaK-rpl3

Feature Metadata

label_name
dnaK-rpl3
feature_type
igs
region
SSC
length_bp
232
variable_sites
38
pi
0.114
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
dnaK-rpl3_p1 TGTCGTACCTAAATCAATTCCT ACAGCCACAATACTCCTCCA 36.4 50.0 54.9 58.3 281-291 bp 1.0 51.1
dnaK-rpl3_p2 TGTCGTACCTAAATCAATTCCTACA ACAGCCACAATACTCCTCCA 36.0 50.0 57.8 58.3 281-291 bp 1.0 51.1
dnaK-rpl3_p3 TGTCGTACCTAAATCAATTCCT CAGCCACAATACTCCTCCAA 36.4 50.0 54.9 57.2 280-290 bp 1.0 51.1
dnaK-rpl3_p4 TTGTCGTACCTAAATCAATTCCT ACAGCCACAATACTCCTCCA 34.8 50.0 55.7 58.3 282-292 bp 1.0 51.0
dnaK-rpl3_p5 TGTCGTACCTAAATCAATTCCT CCCCAATAAACCTACAGCCA 36.4 50.0 54.9 57.2 294-304 bp 1.0 50.9