Hotspot

rrf-psbY

IRb · 71759-72064 · 306 bp

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Pi 0.15
MarkerSeek score 82.80
Primer available yes
Label rrf-psbY

Feature Metadata

label_name
rrf-psbY
feature_type
igs
region
IRb
length_bp
306
variable_sites
62
pi
0.15
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrf-psbY_p1 AACCACACTGATCCATTCCG ATAATGCCCATGAAGCAGCA 50.0 45.0 57.9 57.9 454-465 bp 1.0 90.5
rrf-psbY_p2 CGGAACCACACTGATCCATT ATAATGCCCATGAAGCAGCA 50.0 45.0 57.9 57.9 457-468 bp 1.0 90.5
rrf-psbY_p3 AACCACACTGATCCATTCCG TAATGCCCATGAAGCAGCAA 50.0 45.0 57.9 58.2 453-464 bp 1.0 89.9
rrf-psbY_p4 AACCACACTGATCCATTCCG TGCCCATGAAGCAGCAATTA 50.0 45.0 57.9 58.2 450-461 bp 1.0 89.9
rrf-psbY_p5 CGGAACCACACTGATCCATT TGCCCATGAAGCAGCAATTA 50.0 45.0 57.9 58.2 453-464 bp 1.0 89.9