Hotspot

rps3

LSC · 85741-86400 · 660 bp

Back to Haplophyllum

Pi 0.00606

MarkerSeek score 50.80

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 660
variable_sites: 4
pi: 0.00606
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TGCGGAATTCCTCATTTTTGT	TGATACATACAGGAGTGGAGGA	38.1	45.5	56.7	57.5	778 bp	1.0	41.7
rps3_p2	TGCGGAATTCCTCATTTTTGT	ACATACAGGAGTGGAGGATT	38.1	45.0	56.7	55.2	774 bp	1.0	40.4
rps3_p3	TGCGGAATTCCTCATTTTTGTT	TGATACATACAGGAGTGGAGGA	36.4	45.5	57.4	57.5	778 bp	1.0	40.4
rps3_p4	TTGCGGAATTCCTCATTTTTGT	TGATACATACAGGAGTGGAGGA	36.4	45.5	57.4	57.5	779 bp	1.0	40.4
rps3_p5	TGCGGAATTCCTCATTTTTGT	TGATACATACAGGAGTGGAGG	38.1	47.6	56.7	56.0	778 bp	1.0	40.4