Hotspot

psbC

LSC · 35363-36784 · 1422 bp

Back to Haplophyllum

Pi 0

MarkerSeek score 37.80

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	AAATACGTGCAGCGGAAGAT	ATTTTAAGCATGCGCCTTCG	45.0	45.0	58.0	57.8	1554 bp	1.0	78.3
psbC_p2	TTCCCAGGAAATACGTGCAG	ATTTTAAGCATGCGCCTTCG	50.0	45.0	57.9	57.8	1562 bp	1.0	77.6
psbC_p3	GCAGCGGAAGATCCTGAATT	ATTTTAAGCATGCGCCTTCG	50.0	45.0	58.3	57.8	1546 bp	1.0	75.8
psbC_p4	AGGGTATTCGTGCTTGGATG	ATTTTAAGCATGCGCCTTCG	50.0	45.0	57.7	57.8	1491 bp	1.0	75.8
psbC_p5	AATACGTGCAGCGGAAGATC	ATTTTAAGCATGCGCCTTCG	50.0	45.0	58.4	57.8	1553 bp	1.0	75.0