Hotspot

petL

LSC · 68424-68519 · 96 bp

Back to Haplophyllum

Pi 0.0208

MarkerSeek score 64.50

Primer available yes

Label petL

Feature Metadata

label_name: petL
feature_type: gene
region: LSC
length_bp: 96
variable_sites: 2
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL_p1	TCCTCGTATCGTCCAAAAGTG	CGCAACAGGGGGTTCTTTTT	47.6	50.0	57.8	59.2	223 bp	1.0	60.9
petL_p2	GCTTATCCTCGTATCGTCCA	CGCAACAGGGGGTTCTTTTT	50.0	50.0	56.6	59.2	228 bp	1.0	59.8
petL_p3	TGCTTATCCTCGTATCGTCC	CGCAACAGGGGGTTCTTTTT	50.0	50.0	56.6	59.2	229 bp	1.0	59.8
petL_p4	TGCTTATCCTCGTATCGTCCA	CGCAACAGGGGGTTCTTTTT	47.6	50.0	58.4	59.2	229 bp	1.0	59.3
petL_p5	TCCTCGTATCGTCCAAAAGTG	GTGGAATACCGCAACAGGG	47.6	57.9	57.8	58.5	232 bp	1.0	58.7