Hotspot

atpF

LSC · 11733-13077 · 1345 bp

Back to Haplophyllum

Pi 0.00598

MarkerSeek score 62.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1345
variable_sites: 8
pi: 0.00598
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGTCGGCTTTAATTGTTGCC	ACGCAGTTCTTTATTTTAGTCT	50.0	31.8	58.4	53.8	1471-1480 bp	1.0	40.9
atpF_p2	TCGTCGGCTTTAATTGTTGC	ACGCAGTTCTTTATTTTAGTCT	45.0	31.8	57.4	53.8	1472-1481 bp	1.0	40.9
atpF_p3	GTCGGCTTTAATTGTTGCCA	ACGCAGTTCTTTATTTTAGTCT	45.0	31.8	57.3	53.8	1470-1479 bp	1.0	40.9
atpF_p4	CGTCGGCTTTAATTGTTGCC	ACGCAGTTCTTTATTTTAGTC	50.0	33.3	58.4	52.2	1471-1480 bp	1.0	40.9
atpF_p5	TTCGTCGGCTTTAATTGTTGC	ACGCAGTTCTTTATTTTAGTCT	42.9	31.8	58.1	53.8	1473-1482 bp	1.0	40.9