Hotspot

rps3-rpl22

LSC · 86401-86588 · 188 bp

Back to Haplophyllum

Pi 0.0319

MarkerSeek score 65.20

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 6
pi: 0.0319
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	GTACCAAGGCGGAAACCTAG	GCCGAAGTAAATGAGGGTCC	55.0	55.0	58.0	58.3	303-304 bp	1.0	79.0
rps3-rpl22_p2	GTACCAAGGCGGAAACCTAG	GGTCCTACTGCCGAAAAGAG	55.0	55.0	58.0	58.0	289 bp	0.5	59.7
rps3-rpl22_p3	GGCGGAAACCTAGTGGATTT	GGTCCTACTGCCGAAAAGAG	50.0	55.0	57.9	58.0	282 bp	0.5	58.9
rps3-rpl22_p4	CTTCTTGTAGGCCTTCGCAA	GGTCCTACTGCCGAAAAGAG	50.0	55.0	58.2	58.0	354 bp	0.5	58.4
rps3-rpl22_p5	ATCTTCTTGTAGGCCTTCGC	GGTCCTACTGCCGAAAAGAG	50.0	55.0	57.7	58.0	356 bp	0.5	57.4