Hotspot

psbT-psbN

LSC · 77051-77137 · 87 bp

Back to Haplophyllum

Pi 0.046

MarkerSeek score 78.30

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 87
variable_sites: 4
pi: 0.046
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	47.6	55.0	57.6	58.4	153 bp	1.0	67.5
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	GGCAACCCTCTCAACAACTA	47.6	50.0	57.6	57.4	176 bp	1.0	66.2
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	AGATCCATTCGAGGAACACG	47.6	50.0	57.6	57.4	154 bp	1.0	66.2
psbT-psbN_p4	TCGAGAACCACCTAAAGTTCC	GATCCATTCGAGGAACACGG	47.6	55.0	57.3	58.4	154 bp	1.0	65.3
psbT-psbN_p5	AGTCTCGACTCTAGGGATCA	GATCCATTCGAGGAACACGG	50.0	55.0	56.3	58.4	193 bp	1.0	64.6