Hotspot

rpl20-rps12

LSC · 71620-72388 · 769 bp

Back to Haplophyllum

Pi 0.0065

MarkerSeek score 62.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 769
variable_sites: 5
pi: 0.0065
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTAAGTCGTGAATGAGCCCC	CCGAGGAACATGTACAAGGG	55.0	55.0	58.1	58.0	889-891 bp	1.0	79.8
rpl20-rps12_p2	CTAAGTCGTGAATGAGCCCC	AACCCAAGACAGCCAATCAG	55.0	50.0	58.1	58.1	951-953 bp	1.0	79.3
rpl20-rps12_p3	CTAAGTCGTGAATGAGCCCC	TTACAAAATCCCCCGCTCTG	55.0	50.0	58.1	57.9	926-928 bp	1.0	79.0
rpl20-rps12_p4	CTAAGTCGTGAATGAGCCCC	GTTACAAAATCCCCCGCTCT	55.0	50.0	58.1	57.9	927-929 bp	1.0	79.0
rpl20-rps12_p5	CTAAGTCGTGAATGAGCCCC	CCCAAGACAGCCAATCAGAA	55.0	50.0	58.1	57.8	949-951 bp	1.0	78.3