Hotspot

rps18-rpl20

LSC · 70999-71265 · 267 bp

Back to Haplophyllum

Pi 0.0486

MarkerSeek score 77.40

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 267
variable_sites: 12
pi: 0.0486
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	TGAAAGAAGTGGGTTGACCG	AAGGCGCAGTTGGTTCTTAA	50.0	45.0	58.0	58.0	416-418 bp	1.0	85.6
rps18-rpl20_p2	CCGGTCTTCGAACCAGAAAA	AAGGCGCAGTTGGTTCTTAA	50.0	45.0	58.1	58.0	387-389 bp	1.0	85.3
rps18-rpl20_p3	TGAAAGAAGTGGGTTGACCG	GCGCAGTTGGTTCTTAATCG	50.0	50.0	58.0	57.8	413-415 bp	1.0	84.6
rps18-rpl20_p4	TAGAACTCCCGGTCTTCGAA	AAGGCGCAGTTGGTTCTTAA	50.0	45.0	57.8	58.0	395-397 bp	1.0	84.6
rps18-rpl20_p5	TGAAAGAAGTGGGTTGACCG	AGGCGCAGTTGGTTCTTAAT	50.0	45.0	58.0	57.8	415-417 bp	1.0	84.2