Hotspot

petA-psbJ

LSC · 65066-66181 · 1116 bp

Back to Haplophyllum

Pi 0.00462

MarkerSeek score 64.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1116
variable_sites: 5
pi: 0.00462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	CCGGATTAGGTTCGTCCTTG	50.0	55.0	58.0	58.1	1247-1258 bp	1.0	81.6
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	TCCGGATTAGGTTCGTCCTT	50.0	50.0	58.0	58.1	1248-1259 bp	1.0	81.0
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	CCGGATTAGGTTCGTCCTTG	50.0	55.0	58.2	58.1	1244-1255 bp	1.0	80.5
petA-psbJ_p4	GCATCTGTTGTTTTGGCACA	CCGGATTAGGTTCGTCCTTG	45.0	55.0	57.8	58.1	1212-1223 bp	1.0	80.2
petA-psbJ_p5	TTGGCATCTGTTGTTTTGGC	CCGGATTAGGTTCGTCCTTG	45.0	55.0	57.8	58.1	1215-1226 bp	1.0	80.1