Hotspot

ndhJ-ndhK

LSC · 50940-51046 · 107 bp

Back to Haplophyllum

Pi 0.0187

MarkerSeek score 63.20

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 107
variable_sites: 2
pi: 0.0187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	CCTTGGTAATCGAAGCCCAA	AAATCCCGCCTGAAACCTTT	50.0	45.0	57.9	58.0	246 bp	1.0	79.6
ndhJ-ndhK_p2	CCTTGGTAATCGAAGCCCAA	AATCCCGCCTGAAACCTTTT	50.0	45.0	57.9	58.0	245 bp	1.0	79.6
ndhJ-ndhK_p3	CCTTGGTAATCGAAGCCCAA	ATCCCGCCTGAAACCTTTTT	50.0	45.0	57.9	58.0	244 bp	1.0	79.6
ndhJ-ndhK_p4	TAGCCAAGCAGACAAAGGAC	AATCCCGCCTGAAACCTTTT	50.0	45.0	57.8	58.0	198 bp	1.0	79.3
ndhJ-ndhK_p5	TAGCCAAGCAGACAAAGGAC	ATCCCGCCTGAAACCTTTTT	50.0	45.0	57.8	58.0	197 bp	1.0	79.3