Hotspot

trnE-UUC-trnT-GGU

LSC · 32275-33110 · 836 bp

Back to Haplophyllum

Pi 0.00719
MarkerSeek score 56.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
836
variable_sites
6
pi
0.00719
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 923-938 bp 1.0 79.4
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 921-936 bp 1.0 79.4
trnE-UUC-trnT-GGU_p3 AGAGATGTCCTGAACCGCTA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 902-917 bp 1.0 78.9
trnE-UUC-trnT-GGU_p4 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 900-915 bp 1.0 78.9
trnE-UUC-trnT-GGU_p5 GATGTCCTGAACCGCTAGAC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 920-935 bp 1.0 78.9