Hotspot

trnL-CAA-ycf2

LSC · 149961-150642 · 682 bp

Back to Hansenia

Pi 0.0231

MarkerSeek score 70.80

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: LSC
length_bp: 682
variable_sites: 40
pi: 0.0231
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	ATGGCTTTTCCCGGATGAAA	50.0	45.0	58.0	57.8	826-861 bp	1.0	83.3
trnL-CAA-ycf2_p2	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	825-860 bp	1.0	83.3
trnL-CAA-ycf2_p3	TGAAATGGTAGACACGCGAG	ATGGCTTTTCCCGGATGAAA	50.0	45.0	58.0	57.8	828-863 bp	1.0	83.3
trnL-CAA-ycf2_p4	TGAAATGGTAGACACGCGAG	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	827-862 bp	1.0	83.3
trnL-CAA-ycf2_p5	AAATGGTAGACACGCGAGAC	AAGATGGCTTTTCCCGGATG	50.0	50.0	58.0	58.2	829-864 bp	1.0	83.3