Hotspot

ndhF-rpl32

SSC · 117325-118425 · 1101 bp

Back to Hansenia

Pi 0.022
MarkerSeek score 73.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1101
variable_sites
72
pi
0.022
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGCCGACGGAAAAAGAAGAA CCCAATATCCTTTCCCTTTCCA 45.0 45.5 58.0 57.7 1168-1368 bp 1.0 70.0
ndhF-rpl32_p2 TTGCCGACGGAAAAAGAAGA CCCAATATCCTTTCCCTTTCCA 45.0 45.5 58.0 57.7 1169-1369 bp 1.0 70.0
ndhF-rpl32_p3 AAAAGAAGAAGTCCCGCTCC CCCAATATCCTTTCCCTTTCCA 50.0 45.5 57.8 57.7 1158-1358 bp 1.0 68.9
ndhF-rpl32_p4 AAGAAGAAGTCCCGCTCCTA CCCAATATCCTTTCCCTTTCCA 50.0 45.5 57.8 57.7 1156-1356 bp 1.0 68.5
ndhF-rpl32_p5 AGAAGAAGTCCCGCTCCTAT CCCAATATCCTTTCCCTTTCCA 50.0 45.5 57.5 57.7 1155-1355 bp 1.0 66.7