Hotspot

ndhC-trnV-UAC

LSC · 54405-55669 · 1265 bp

Back to Hansenia

Pi 0.0294
MarkerSeek score 70.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1265
variable_sites
75
pi
0.0294
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 31.8 55.0 53.9 58.1 1046-1324 bp 1.0 48.9
ndhC-trnV-UAC_p2 TGCCAAAATAGGAATAAGACTTGA CCGAGAAAGTCTACGGTTCG 33.3 55.0 56.3 58.1 1095-1373 bp 1.0 48.7
ndhC-trnV-UAC_p3 TGCCAAAATAGGAATAAGACTTG CCGAGAAAGTCTACGGTTCG 34.8 55.0 54.9 58.1 1095-1373 bp 1.0 48.7
ndhC-trnV-UAC_p4 ATGCCAAAATAGGAATAAGACTTGA CCGAGAAAGTCTACGGTTCG 32.0 55.0 56.8 58.1 1096-1374 bp 1.0 48.7
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAAGACTTGAT CCGAGAAAGTCTACGGTTCG 32.0 55.0 56.8 58.1 1095-1373 bp 1.0 48.7