Hotspot

rps4-trnT-UGU

LSC · 50099-50437 · 339 bp

Back to Hansenia

Pi 0.00983

MarkerSeek score 63.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 339
variable_sites: 9
pi: 0.00983
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	ACTTCCGACTCTAGGCCTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	485-512 bp	1.0	81.2
rps4-trnT-UGU_p2	ACTTCCGACTCTAGGCCTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	453-480 bp	1.0	80.5
rps4-trnT-UGU_p3	ACTTCCGACTCTAGGCCTTT	TCGATTATGTGAGCAGCCTG	50.0	50.0	58.1	57.8	510-537 bp	1.0	80.3
rps4-trnT-UGU_p4	GTTAGTCCCGGTAAAGCTCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.7	57.9	460-487 bp	1.0	79.4
rps4-trnT-UGU_p5	GTTAGTCCCGGTAAAGCTCC	TCGATTATGTGAGCAGCCTG	55.0	50.0	57.7	57.8	485-512 bp	1.0	78.5