Hotspot

rpoC2-rpoC1

LSC · 23549-23759 · 211 bp

Back to Hansenia

Pi 0.0325

MarkerSeek score 65.10

Primer available yes

Label rpoC2-rpoC1

Feature Metadata

label_name: rpoC2-rpoC1
feature_type: igs
region: LSC
length_bp: 211
variable_sites: 18
pi: 0.0325
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rpoC1_p1	CCCGTTCTGCCATAAGTACC	GGGTTTTGTCAGGCCTGTTA	55.0	50.0	58.1	58.0	257-298 bp	1.0	84.9
rpoC2-rpoC1_p2	GCGGTTCCGTCTATCACTTT	GGGTTTTGTCAGGCCTGTTA	50.0	50.0	58.0	58.0	297-338 bp	1.0	84.6
rpoC2-rpoC1_p3	TGTGAAAGACCAGATTGGCC	GGGTTTTGTCAGGCCTGTTA	50.0	50.0	58.1	58.0	275-316 bp	1.0	84.2
rpoC2-rpoC1_p4	TAAGTCGTTTCATGGCGGTT	GGGTTTTGTCAGGCCTGTTA	45.0	50.0	57.8	58.0	311-352 bp	1.0	83.0
rpoC2-rpoC1_p5	GCGGTTCCGTCTATCACTTT	ATACAGGGGTTTTGTCAGGC	50.0	50.0	58.0	57.8	303-344 bp	1.0	82.8