Hotspot

trnK-UUU-rps16

LSC · 6529-7129 · 601 bp

Back to Hansenia

Pi 0.0515
MarkerSeek score 83.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
601
variable_sites
88
pi
0.0515
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCCCTCGCTTCATCCAAATG AAAGGCGGAGGTTTTTAAGT 50.0 40.0 58.0 55.5 640-995 bp 1.0 71.3
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC AAAGGCGGAGGTTTTTAAGT 55.0 40.0 57.9 55.5 657-1012 bp 1.0 71.0
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC AAAGGCGGAGGTTTTTAAGT 55.0 40.0 57.9 55.5 656-1011 bp 1.0 71.0
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AAAGGCGGAGGTTTTTAAGT 50.0 40.0 58.2 55.5 596-951 bp 1.0 70.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AAAGGCGGAGGTTTTTAAGT 50.0 40.0 58.2 55.5 595-950 bp 1.0 70.3