Hotspot

rps18

LSC · 72000-72305 · 306 bp

Back to Hamamelis

Pi 0

MarkerSeek score 20.00

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 306
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 20.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	GTGAAACTCGGGTTCAGGTT	CGTTTGTGGATCACTCGGAT	50.0	50.0	58.0	58.0	1469-1474 bp	1.0	80.2
rps18_p2	GTGAAACTCGGGTTCAGGTT	GAGTTCCCCGGAGAATGAAC	50.0	55.0	58.0	58.0	1253-1258 bp	1.0	80.1
rps18_p3	GTGAAACTCGGGTTCAGGTT	GTTCCCCGGAGAATGAACTC	50.0	55.0	58.0	58.0	1251-1256 bp	1.0	80.1
rps18_p4	TGGAAATGTGGGTCTAGGGT	CGTTTGTGGATCACTCGGAT	50.0	50.0	57.9	58.0	1410-1415 bp	1.0	80.1
rps18_p5	TGGAAATGTGGGTCTAGGGT	GTTCCCCGGAGAATGAACTC	50.0	55.0	57.9	58.0	1192-1197 bp	1.0	80.0