Hotspot

rpl22

LSC · 87581-88000 · 420 bp

Back to Hamamelis

Pi 0.00317

MarkerSeek score 32.30

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 420
variable_sites: 2
pi: 0.00317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TCTTCACCTAAGGATATATTTTTCA	TAAATTTCCGGGGACATGCG	28.0	50.0	53.8	58.6	548 bp	1.0	40.3
rpl22_p2	TCTTCACCTAAGGATATATTTTTCAA	TAAATTTCCGGGGACATGCG	26.9	50.0	54.5	58.6	548 bp	1.0	40.3
rpl22_p3	TTCTTCACCTAAGGATATATTTTTCA	TAAATTTCCGGGGACATGCG	26.9	50.0	54.5	58.6	549 bp	1.0	40.3
rpl22_p4	CTTCACCTAAGGATATATTTTTCA	TAAATTTCCGGGGACATGCG	29.2	50.0	52.3	58.6	547 bp	1.0	40.3
rpl22_p5	TCTTCACCTAAGGATATATTTTTCA	CTAAATTTCCGGGGACATGC	28.0	50.0	53.8	56.8	549 bp	1.0	40.3