Hotspot

rbcL

LSC · 58921-60348 · 1428 bp

Back to Hamamelis

Pi 0.0014

MarkerSeek score 47.10

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 3
pi: 0.0014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGAGTTGTAGGGAGGGACTT	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.3	58.4	1492 bp	1.0	71.4
rbcL_p2	TGAGTTGTAGGGAGGGACTT	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.3	58.4	1493 bp	1.0	71.4
rbcL_p3	TGAGTTGTAGGGAGGGACTT	AATTGCAAAGGGAACGAACG	50.0	45.0	57.3	57.6	1480 bp	1.0	71.1
rbcL_p4	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.1	58.4	1495 bp	1.0	69.6
rbcL_p5	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.1	58.4	1496 bp	1.0	69.6