Hotspot

psbC

LSC · 36968-38389 · 1422 bp

Back to Hamamelis

Pi 0

MarkerSeek score 35.90

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GTGCAGCAGAAGATCCTGAA	ACCTGATCCGGATCCGAATA	50.0	50.0	57.9	57.7	1591 bp	1.0	76.7
psbC_p2	AAATCCGTGCAGCAGAAGAT	ACCTGATCCGGATCCGAATA	45.0	50.0	57.9	57.7	1597 bp	1.0	76.6
psbC_p3	TTCGTTTCCCAGGAAATCCG	ACCTGATCCGGATCCGAATA	50.0	50.0	58.2	57.7	1610 bp	1.0	76.1
psbC_p4	GTGCAGCAGAAGATCCTGAA	TATGACCTGATCCGGATCCG	50.0	55.0	57.9	58.5	1595 bp	1.0	75.5
psbC_p5	AAATCCGTGCAGCAGAAGAT	TATGACCTGATCCGGATCCG	45.0	55.0	57.9	58.5	1601 bp	1.0	75.4