Hotspot

rps18-rpl20

LSC · 72306-72596 · 291 bp

Back to Hamamelis

Pi 0.0115

MarkerSeek score 61.80

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 291
variable_sites: 5
pi: 0.0115
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	TTGAAAGAACCGAGTCGACC	CACGATCTGTACAAGAGGCA	50.0	50.0	57.9	57.3	450-455 bp	1.0	75.4
rps18-rpl20_p2	TTGAAAGAACCGAGTCGACC	ACACGATCTGTACAAGAGGC	50.0	50.0	57.9	57.3	451-456 bp	1.0	75.4
rps18-rpl20_p3	AGTCGACCGCTAGAACTACT	CACGATCTGTACAAGAGGCA	50.0	50.0	57.6	57.3	438-443 bp	1.0	73.4
rps18-rpl20_p4	AGTCGACCGCTAGAACTACT	ACACGATCTGTACAAGAGGC	50.0	50.0	57.6	57.3	439-444 bp	1.0	73.4
rps18-rpl20_p5	GTCGACCGCTAGAACTACTG	CACGATCTGTACAAGAGGCA	55.0	50.0	57.5	57.3	437-442 bp	1.0	72.9