Hotspot

ycf4-cemA

LSC · 64274-65165 · 892 bp

Back to Hamamelis

Pi 0.00149

MarkerSeek score 48.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 892
variable_sites: 2
pi: 0.00149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.1	58.1	1003-1011 bp	1.0	79.4
ycf4-cemA_p2	AAAAAGCTGCTGAATTGGCC	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	1017-1025 bp	1.0	78.8
ycf4-cemA_p3	AAAGCTGCTGAATTGGCCTA	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	1015-1023 bp	1.0	78.4
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	GAGAGAGAGATCCACCAGGG	45.0	60.0	58.1	58.3	1009-1017 bp	1.0	77.7
ycf4-cemA_p5	AAAAAGCTGCTGAATTGGCC	GAGAGAGAGATCCACCAGGG	45.0	60.0	57.8	58.3	1023-1031 bp	1.0	77.0