Hotspot

psbK-psbI

LSC · 8583-8965 · 383 bp

Back to Hamamelis

Pi 0.00348

MarkerSeek score 43.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 383
variable_sites: 2
pi: 0.00348
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CCAAATTACCCGAGGCCTAC	TCTTCACGTCCAGGATTACG	55.0	50.0	58.0	57.1	606 bp	1.0	72.5
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	520 bp	1.0	72.4
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	517 bp	1.0	70.4
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	518 bp	1.0	70.4
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	519 bp	1.0	70.4