Hotspot

rps16-trnQ-UUG

LSC · 6301-7976 · 1676 bp

Back to Hamamelis

Pi 0.00159

MarkerSeek score 47.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1676
variable_sites: 4
pi: 0.00159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.0	58.0	1812-1814 bp	1.0	80.2
rps16-trnQ-UUG_p2	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1754-1756 bp	1.0	79.6
rps16-trnQ-UUG_p3	ATGTCTTTCAAGTCGCACGT	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1814-1816 bp	1.0	79.1
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1805-1807 bp	1.0	79.1
rps16-trnQ-UUG_p5	ATGTCTTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1756-1758 bp	1.0	78.6